Publications

  1. Nowoshilow S, Schloissnig S, …, Tanaka EM, Myers EM (2017). The axolotl genome and the evolution of key tissue formation regulators. Nature. 554(7690):50-55.
  2. Oliveira CR, …, Tanaka EM. (2017) Pseudotyped baculovirus is an effective gene expression tool for studying molecular function during axolotl limb regeneration. Dev Biol S0012-1606(17)30438-4
  3. Fei JF, Schuez M, …, Tanaka EM. (2017). Efficient gene knockin in axolotl and its use to test the role of satellite cells in limb regeneration. . Proc Natl Acad Sci U S A 114:12501-12506.
  4. Currie JD, Kawaguchi A …, Tanaka EM. (2016). Live Imaging of Axolotl Digit Regeneration Reveals Spatiotemporal Choreography of Diverse Connective Tissue Progenitor Pools. Dev Cell 39:411-423.
  5. Nacu E, Shabanova E, …, Tanaka EM. (2016). SHH and FGF8 substitute anterior/posterior tissue interactions to induce limb regeneration. Nature 533:407-410.
  6. Gabisonia, K., Prosdocimo, G., Aquaro, G.D., Carlucci, L., Zentilin, L., Secco, I., Ali, A., Braga, L., Gorgodze, N., Bernini, F., Burchielli, S., Collesi, C., Zandonà, L., Sinagra, G., Piacenti, M., Zacchigna, S., Bussani, R., Recchia, F.A., Giacca, M. (2019). MicroRNA therapy stimulates uncontrolled cardiac repair after myocardial infarction in pigs. Nature, in press
  7. Zacchigna, S., Martinelli, V., Moimas, S., Colliva, A., Anzini, M., Nordio, A., Costa, A., Rehman, M., Vodret, S., Pierro, C., Colussi, G., Zentilin, L., Gutierrez, M.I., Dirkx, E., Long, C., Sinagra, G., Klatzmann, D., Giacca, M. (2018). Paracrine effect of regulatory T cells promotes cardiomyocyte proliferation during pregnancy and after myocardial infarction. Nat Commun 9, 2432.
  8. Lesizza, P., Prosdocimo, G., Martinelli, V., Sinagra, G., Zacchigna, S., Giacca, M. (2017). Single-Dose Intracardiac Injection of Pro-Regenerative MicroRNAs Improves Cardiac Function After Myocardial Infarction. Circ Res 120, 1298-1304.
  9. Bortolotti, F., Ruozi, G., Falcione, A., Doimo, S., Dal Ferro, M., Lesizza, P., Zentilin, L., Banks, L., Zacchigna, S., Giacca, M. (2017). In Vivo Functional Selection Identifies Cardiotrophin-1 as a Cardiac Engraftment Factor for Mesenchymal Stromal Cells. Circulation 136, 1509-1524.
  10. Eulalio, A., Mano, M., Dal Ferro, M., Zentilin, L., Sinagra, G., Zacchigna, S., Giacca, M. (2012). Functional screening identifies miRNAs inducing cardiac regeneration. Nature 492, 376-81.
  11. Muñoz-Martín, N., Sierra, R., Schimmang, T., del Campo, C. V., & Torres, M. (2019). Myc is dispensable for cardiomyocyte development but rescues Mycn-deficient hearts through functional replacement and cell competition. Development, 146(3), dev170753.
  12. Díaz-Díaz, C., de Manuel, L. F., Jimenez-Carretero, D., Montoya, M. C., Clavería, C., & Torres, M. (2017). Pluripotency surveillance by Myc-driven competitive elimination of differentiating cells. Developmental Cell, 42(6), 585-599.
  13. del Campo, C. V., Clavería, C., Sierra, R., & Torres, M. (2014). Cell competition promotes phenotypically silent cardiomyocyte replacement in the mammalian heart. Cell reports, 8(6), 1741-1751.
  14. Clavería, C., Giovinazzo, G., Sierra, R., & Torres, M. (2013). Myc-driven endogenous cell competition in the early mammalian embryo. Nature, 500(7460), 39.
  15. Fernández-Jiménez, R., Galán-Arriola, C., Sánchez-González, J., Agüero, J., López-Martín, G. J., Gomez-Talavera, S., … & Ibanez, B. (2017). Effect of ischemia duration and protective interventions on the temporal dynamics of tissue composition after myocardial infarction. Circulation Research, 121(4), 439-450.
  16. Mosqueira D, Mannhardt I, Bhagwan JR, Lis-Slimak K, Katili P, Scott E, Hassan M, Prondzynski M, Harmer SC, Tinker A, Smith JGW, Carrier L, Williams PM, Gaffney D, Eschenhagen T, Hansen A, Denning C (2018) CRISPR/Cas9 editing in human pluripotent stem cell-cardiomyocytes highlights arrhythmias, hypocontractility, and energy depletion as potential therapeutic targets for hypertrophic cardiomyopathy.Eur Heart J 39:3879-3892
  17. Neidig LE, Weinberger F, Palpant NJ, Mignone J, Martinson AM, Sorensen DW, Bender I, Nemoto N, Reinecke H, Pabon L, Molkentin JD, Murry CE, van Berlo JH (2018) Evidence for minimal cardiogenic potential of stem cell antigen 1-positive cells in the adult mouse heart. Circulation 138: 2960-2962
  18. Breckwoldt K, Letuffe-Brenière D, Mannhardt I, Schulze T, Ulmer B, Werner T, Benzin A, Klampe B, Reinsch MC, Laufer S, Shibamiya A, Prondzynski M, Mearini G, Schade D, Fuchs S, Neuber C, Krämer E, Saleem U, Schulze ML, Rodriguez ML, Eschenhagen T, Hansen A (2017) Differentiation of cardiomyocytes and generation of human engineered heart tissue. Nat Protoc 12:1177-1197
  19. Weinberger F, Breckwoldt K, Pecha S, Kelly A, Geertz B, Starbatty J, Yorgan T, Cheng KH, Lessmann K, Stolen T, Scherrer-Crosbie M, Smith G, Reichenspurner H, Hansen A, Eschenhagen T (2016) Cardiac repair in guinea pigs with human engineered heart tissue from induced pluripotent stem cells. Sci Transl Med 8: 363ra148.
  20. Weinberger F, Mehrkens D, Friedrich FW, Stubbendorff M, Hua X, Müller JC, Schrepfer S, Evans SM, Carrier L, Eschenhagen T (2012) Localization of Islet-1-positive cells in the healthy and infarcted adult murine heart. Circ Res 110:1303-10.
  21. Sánchez-Iranzo, H., Galardi-Castilla, M., Sanz-Morejón, A., González-Rosa, J. M., Costa, R., Ernst, A Sainz de Aja J,  Langa L,  Mercader N. (2018). Transient fibrosis resolves via fibroblast inactivation in the regenerating zebrafish heart. Proceedings of the National Academy of Sciences115(16), 4188-4193.
  22. Sánchez-Iranzo, H., Galardi-Castilla, M., Minguillón, C., Sanz-Morejón, A., González-Rosa, J. M., Felker, A Ernst A, Guzmán-Martínez G, Mosimann C, Mercader N. (2018). Tbx5a lineage tracing shows cardiomyocyte plasticity during zebrafish heart regeneration. Nature communications9(1), 428.
  23. Bednarek D, González-Rosa JM, Guzmán-Martínez G, Gutiérrez-Gutiérrez Ó, Aguado T, Sánchez-Ferrer C, Marques IJ, Galardi-Castilla M, de Diego I, Gómez MJ, Cortés A, Zapata A, Jiménez-Borreguero LJ, Mercader N, Flores I. (2015). Telomerase Is Essential for Zebrafish Heart Regeneration. Cell Rep.; 12:1691-703. (N Mercader co-corresponding author)
  24. González-Rosa JM, Mercader N. (2012). Cryoinjury as a myocardial infarction model for the study of cardiac regeneration in the zebrafish. Nat Protoc. Mar 29;7(4):782-8.
  25. Gonzalez-Rosa, J.M., Martin, V., Peralta, M., Torres, M., and Mercader, N. (2011) Extensive scar formation and regression during heart regeneration after cryoinjury in zebrafish. Development 138, 1663-1674.
  26. D’Uva, G., Aharonov, A., Lauriola, M., Kain, D., Yahalom-Ronen, Y., Carvalho, S., … & Tzahor, E. (2015). ERBB2 triggers mammalian heart regeneration by promoting cardiomyocyte dedifferentiation and proliferation. Nature cell biology17(5), 627.
  27. Bassat, E., Mutlak, Y. E., Genzelinakh, A., Shadrin, I. Y., Umansky, K. B., Yifa, O., … & Tzahor, E. (2017). The extracellular matrix protein agrin promotes heart regeneration in mice. Nature547(7662), 179.
  28. Tzahor, E. (2007). Wnt/β-catenin signaling and cardiogenesis: timing does matter. Developmental Cell13(1), 10-13.
  29. Tzahor, E. (2015). Cardiac and craniofacial development and regeneration. The FASEB Journal29(1_supplement), 211-2.
  30. Tzahor, E., & Poss, K. D. (2017). Cardiac regeneration strategies: staying young at heart. Science356(6342), 1035-1039.
  31. Gladka, M. M., Molenaar, B., De Ruiter, H., Van Der Elst, S., Tsui, H., Versteeg, D., … & van Rooij, E. (2018). Single-cell sequencing of the healthy and diseased heart reveals cytoskeleton-associated protein 4 as a new modulator of fibroblasts activation. Circulation138(2), 166-180.
  32. Lacraz GPA, Junker JP, Gladka MM, Molenaar B, Scholman KT, Vigil-Garcia M, Versteeg D, de Ruiter H, Vermunt MW, Creyghton MP, Huibers MMH, de Jonge N, van Oudenaarden A, van Rooij E. (2017) Tomo-seq Identifies SOX9 as a Key Regulator of Cardiac Fibrosis During Ischemic Injury. Circulation. Jul 19
  33. Johansen AK, Molenaar B, Versteeg D, Leitoguinho AR, Demkes CJ, Spanjaard B, de Ruiter H, Akbari Moqadam FA, Kooijman L, Zentilin L, Giacca M, van Rooij E. (2017) Postnatal Cardiac Gene-Editing Using CRISPR/Cas9 with AAV9-Mediated Delivery of Short Guide RNAs Results in Mosaic Gene Disruption. Circ Res. Aug 29
  34. van Rooij E. (2016) Cardiac Repair after Myocardial Infarction. N Engl J Med. 7;374(1):85-7
  35. Montgomery, R. L., Yu, G., Latimer, P. A., Stack, C., Robinson, K., Dalby, C. M., … & van Rooij, E. (2014). MicroRNA mimicry blocks pulmonary fibrosis. EMBO molecular medicine6(10), 1347-1356.
  36. Kormann, M. S., G. Hasenpusch, Aneja, G. Nica, A. W. Flemmer, S. Herber-Jonat, M. Huppmann, L. E. Mays, M. Illenyi, A. Schams, M. Griese, I. Bittmann, R. Handgretinger, D. Hartl, J. Rosenecker and C. Rudolph (2011). Expression of therapeutic proteins after delivery of chemically modified mRNA in mice. Nat Biotechnol 29(2): 154-157.
  37. Ottersbach, A., O. Mykhaylyk, A. Heidsieck, D. Eberbeck, S. Rieck, K. Zimmermann, M. Breitbach, B. Engelbrecht, T. Brugmann, M. Hesse, A. Welz, P. Sasse, D. Wenzel, Plank, B. Gleich, M. Holzel, W. Bloch, A. Pfeifer, B. K. Fleischmann and W. Roell (2018). Improved heart repair upon myocardial infarction: Combination of magnetic nanoparticles and tailored magnets strongly increases engraftment of myocytes. Biomaterials 155: 176-190.
  38. Schrom, E., M. Huber, Aneja, C. Dohmen, D. Emrich, J. Geiger, G. Hasenpusch, A. Herrmann-Janson, V. Kretzschmann, O. Mykhailyk, T. Pasewald, P. Oak, A. Hilgendorff, D. Wohlleber, H. G. Hoymann, D. Schaudien, C. Plank, C. Rudolph and R. Kubisch-Dohmen (2017). Translation of Angiotensin-Converting Enzyme 2 upon Liver- and Lung-Targeted Delivery of Optimized Chemically Modified mRNA. Mol Ther Nucleic Acids 7: 350-365.
  39. Vosen, S., S. Rieck, A. Heidsieck, O. Mykhaylyk, K. Zimmermann, W. Bloch, D. Eberbeck, Plank, B. Gleich, A. Pfeifer, B. K. Fleischmann and D. Wenzel (2016). Vascular Repair by Circumferential Cell Therapy Using Magnetic Nanoparticles and Tailored Magnets. ACS Nano 10(1): 369-376.
  40. Vosen, S., S. Rieck, A. Heidsieck, O. Mykhaylyk, K. Zimmermann, C. Plank, B. Gleich, A. Pfeifer, B. K. Fleischmann and D. Wenzel (2016). Improvement of vascular function by magnetic nanoparticle-assisted circumferential gene transfer into the native endothelium. J Control Release 241: 164-173.
  41. Letelier J*, Terriente J*, et al (2018) The evolutionary emergence of the rac3b/rfng/sgca regulatory cluster refined mechanisms for hindbrain boundaries formation. Proc Natl Acad Sci (*Co-authorship); doi: 10.1073/pnas.1719885115
  42. Cornet C, Di Donato V*, Terriente J*. (2018) Combining the zebrafish model with the CRISPR/Cas9 system: towards a more efficient drug discovery pipeline. Frontiers in Pharmacology (*equal contribution (Review)); doi: 10.3389/fphar.2018.00703
  43. Cornet C, Calzolari S, Miñana-Prieto R, Dyballa S, van Doornmalen E, Rutjes H, Savy T, D´Amico D, Terriente J. (2017) ZeGlobalTox: An Innovative Approach to Address Organ Drug Toxicity Using Zebrafish. Int J Mol Sci. doi: 10.3390/ijms18040864
  44. D’Amico D, Estivill X, Terriente J. (2015) Switching to zebrafish neurobehavioral models: The obsessive–compulsive disorder paradigm. Eur J Pharmacol. doi: 10.1016/j.ejphar.2015.03.027
  45. Terriente J, Calzolari S, Pujades C (2014) Cell segregation in the vertebrate hindbrain relies in an actomyosin cable located at interhombomeric boundaries. EMBO J. doi:10.1002/embj.201386003.
  46. Längin M, Mayr T, Reichart B, Michel S, Buchholz S, Guethoff S, Dashkevich A, Baehr A, Egerer S, Bauer A, Mihalj M, Panelli A, Issl L, Ying J, Fresch AK, Buttgereit I, Mokelke M, Radan J, Werner F, Lutzmann I, Steen S, Sjöberg T, Paskevicius A, Qiuming L, Sfriso R, Rieben R, Dahlhoff M, Kessler B, Kemter E, Klett K, Hinkel R, Kupatt C, Falkenau A, Reu S, Ellgass R, Herzog R, Binder U, Wich G, Skerra A, Ayares D, Kind A, Schönmann U, Kaup FJ, Hagl C, Wolf E, Klymiuk N, Brenner P, Abicht JM. Consistent success in life-supporting porcine cardiac xenotransplantation. Nature. doi: 10.1038/s41586-018-0765-z. 2018
  47. Hinkel R, Hoewe A, Renner S, Ng J, Lee S, Klett K, Kaczmarek V, Moretti A, Laugwitz KL, Skroblin P, Mayr M, Milting H, Dendorfer A, Reichart B, Wolf E, Kupatt C. Diabetes Mellitus-Induced Microvascular Destabilization in the Myocardium. J Am Coll Cardiol. 69(2):131-143. 2017
  48. Hinkel R, Lange P, Petersen B, Gottlieb E, Ng JK, Finger S, Horstkotte J, Lee S, Thormann M, Knorr M, El-Aouni C, Boekstegers P, Reichart B, Wenzel P, Niemann H, Kupatt C. Heme Oxygenase-1 Gene Therapy Provides Cardioprotection Via Control of Post-Ischemic Inflammation: An Experimental Study in a Pre-Clinical Pig Model. J Am Coll Cardiol. 66(2):154-65. 2015
  49. Hinkel R, Trenkwalder T, Petersen B, Husada W, Gesenhues F, Lee S, Hannappel E, Bock-Marquette I, Theisen D, Leitner L, Boekstegers P, Cierniewski C, Müller OJ, le Noble F, Adams RH, Weinl C, Nordheim A, Reichart B, Weber C, Olson E, Posern G, Deindl E, Niemann H, Kupatt C. MRTF-A controls vessel growth and maturation by increasing the expression of CCN1 and CCN2. Nat Commun. 5:3970. 2014
  50. Hinkel R, Penzkofer D, Zühlke S, Fischer A, Husada W, Xu QF, Baloch E, van Rooij E, Zeiher AM, Kupatt C, Dimmeler S. Inhibition of microRNA-92a protects against ischemia/reperfusion injury in a large-animal model. Circulation. 128(10):1066-75. 2013.
  51. Matteucci M, Casieri V, Gabisonia K, Aquaro GD, Agostini S, Pollio G, Diamanti D, Rossi M, Travagli M, Porcari V, Recchia FA, Lionetti V. (2016) Magnetic resonance imaging of infarct-induced canonical wingless/integrated (Wnt)/β-catenin/T-cell factor pathway activation, in-vivo. Cardiovasc Res. 112:645-655
  52. Woitek F, Zentilin L, Hoffman NE, Powers JC, Ottiger I, Parikh S, Kulczycki AM, Hurst M, Ring N, Wang T, Shaikh F, Gross P, Singh H, Kolpakov MA, Linke A, Houser SR, Rizzo V, Sabri A, Madesh M, Giacca M, Recchia FA. (2015) Intracoronary cytoprotective gene therapy: A study of VEGF-B167 in a pre-clinical animal model of dilated cardiomyopathy. J Am Coll Cardiol. 66:139-53.
  53. Aquaro GD, Frijia F, Positano V, Menichetti L, Santarelli MF, Ardenkjaer-Larsen JH, Wiesinger F, Lionetti V, Romano SL, Bianchi G, Neglia D, Giovannetti G, Schulte RF, Recchia FA, Landini L, Lombardi M. (2013) 3D CMR mapping of metabolism by hyperpolarized 13C-pyruvate in ischemia-reperfusion. JACC Cardiovasc Imaging ;6:743-4.
  54. Simioniuc A, Campan M, Lionetti V, Marinelli M, Aquaro GD, Cavallini C, Valente S, Di Silvestre D, Cantoni S, Bernini B, Simi C, Pardini S, Mauri P, Neglia D, Ventura C, Pasquinelli G, Recchia FA. (2011) Placental stem cells pre-treated with hyaluronan-butyric-retinoic ester to cure infarcted pig hearts: a multimodal study. Cardiovasc Res;90:546-56.
  55. Campan M, Lionetti V, Aquaro GD, Forini F, Matteucci M, Vannucci L, Chiuppesi F, Di Cristofano C, Faggioni M, Maioli M, Barile L, Messina E, Lombardi M, Pucci A, Pistello M, Recchia FA. (2011) Ferritin as a reporter gene for in-vivo tracking of stem cells by 1.5T cardiac MRI in a rat model of myocardial infarction. Am J Physiol; 300:H2238-50.
  56. rento C, Bernardo ME, Nagler A, Kuçi S, Bornhäuser M, Koehl U, Strunk D, Galleu A, Sanchez-Guijo F, Gaipa G, Introna M, Bukauskas A, Le Blanc K, Apperley J, Roelofs H, Van Campenhout A, Beguin Y, Kuball J, Lazzari L, Avanzini MA, Fibbe W, Chabannon C, Bonini C, Dazzi F. (2018) Manufacturing Mesenchymal Stromal Cells for the Treatment of Graft-versus-Host Disease: A Survey among Centers Affiliated with the European Society for Blood and Marrow Transplantation Biol Blood Marrow Transplant. 2018 Nov;24(11):2365-2370. doi: 10.1016/j.bbmt.2018.07.015.
  57. Tischer S, Priesner C, Heuft HG, Goudeva L, Mende W, Barthold M, Kloeß S, Arseniev L, Aleksandrova K, Maecker-Kolhoff B, Blasczyk R, Koehl U, Eiz-Vesper B. (2014) Rapid generation of clinical-grade antiviral T cells: selection of suitable T-cell donors and GMP-compliant manufacturing of antiviral T cells. J Transl Med. 16;12:336. doi: 10.1186/s12967-014-0336-5.
  58. Kloss S, Oberschmidt O, Morgan M, Dahlke J, Arseniev L, Huppert V, Granzin M, Gardlowski T, Matthies N, Soltenborn S, Schambach A, Koehl U. (2017) Optimization of Human NK Cell Manufacturing: Fully Automated Separation, Improved Ex Vivo Expansion Using IL-21 with Autologous Feeder Cells, and Generation of Anti-CD123-CAR-Expressing Effector Cells. Hum Gene Ther.
  59. Koehl U, Kaberer C, Spanholtz J, Lee DA, Miller JS, Cooley S, Lowdell M, Uharek L, Klingemann H, Curti A, Leung W, Alici E. (2016) Advances in clinical NK cell studies: Donor selection, manufacturing and quality control. OncoImmunology 2015 Nov 11;5(4):e1115178.
  60. Aleksandrova K, Leise J, Priesner C, Melk A, Kubaink F, Abken H, Hombach A, Aktas M, Essl M, Bürger I, Kaiser A, Rauser G, Jurk M, Goudeva L, Glienke W, Arseniev L, Esser R, Koehl U. (2019) Functionality and Cell Senescence of CD4/ CD8-Selected CD20 CAR T Cells Manufactured Using the Automated CliniMACS Prodigy® Platform. Transfusion Medicine and Hematotherapy, Vol.46, No. 1.